Determination of Borrelia surface lipoprotein anchor topology by surface proteolysis.
نویسندگان
چکیده
We used a surface trypsinolysis assay to probe accessibility of the membrane-proximal N-terminal tether peptides of Borrelia surface lipoproteins OspA and Vsp1. Our findings with both wild-type and mutant proteins are only compatible with the anchoring of these surface lipoproteins in the outer leaflet of the outer spirochetal membrane.
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ورودعنوان ژورنال:
- Journal of bacteriology
دوره 193 22 شماره
صفحات -
تاریخ انتشار 2011